Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line

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dc.authorid0000-0002-6424-7411en_US
dc.authorscopusid57366670900en_US
dc.contributor.authorKaragülle, Onur Olgaç
dc.contributor.authorGök Yurttaş, Asiye
dc.date.accessioned2022-06-06T07:02:22Z
dc.date.available2022-06-06T07:02:22Z
dc.date.issued2022en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Biyokimya Ana Bilim Dalıen_US
dc.description.abstractObjective: We aimed to examine the potential anticancer effects of ozone applied after chemotherapeutic treatment with different concentrations of doxorubicin in Luminal-A subtype of human breast cancer cell line (MCF-7) and compare the results with effects on L929 fibroblast cell line. Methods: Both cell lines were incubated with increasing doses of doxorubicin (1-50 μM) for 24 h at 37°C. Then, half of groups were incubated with 30 μg/mL ozone for 25 min as combination groups. Cell viability was analyzed by MTT assay, apoptosis by flow cytometry, and levels of tumor necrosis factor alpha, transforming growth factor beta, and matrix metalloproteinase-2 and MMP-9 by immunocytochemistry. Results: Doxorubicin + ozone treatment enhanced viability of L929 (p<0.01) but reduced viability of MCF-7 compared to only doxorubicin-applied cells without ozone treatment (p<0.001). This combined treatment also enhanced apoptotic effect of doxorubicin on MCF-cells (p<0.001), but not on L929. It significantly increased all protein levels of L929 compared with those of other groups (p<0.05 for tumor necrosis factor alpha and MMP-2; p<0.01 for transforming growth factor beta and MMP-9). This treatment reversed the effect of doxorubicin on tumor necrosis factor alpha levels and considerably reduced MMP-2 and MMP-9 levels of MCF-7 compared with those of control group (p<0.01 and p<0.001, respectively). Conclusion: Ozone treatment potentiated the apoptotic and anticancer activities of doxorubicin in MCF-7 cells and showed repairing and healing effect on healthy fibroblast cells, which were damaged from cytotoxic effects of chemotherapeutic agent. MCF-7 cells may acquire sensitivity against the doxorubicin combined with ozone treatment through activating tumor necrosis factor alpha, MMP-2, and MMP-9 expressions.en_US
dc.identifier.citationKaragülle, O. O. & Gök Yurttaş, A. (2022). Ozone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell line. Revista da Associacao Medica Brasileira, 68(4), 507–513. https://doi.org/10.1590/1806-9282.20211193en_US
dc.identifier.doi10.1590/1806-9282.20211193en_US
dc.identifier.endpage513en_US
dc.identifier.issue4en_US
dc.identifier.pmidPMID: 35649075en_US
dc.identifier.scopus2-s2.0-85131270939en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage507en_US
dc.identifier.urihttps://doi.org/10.1590/1806-9282.20211193
dc.identifier.urihttps://hdl.handle.net/20.500.13055/223
dc.identifier.volume68en_US
dc.identifier.wosWOS:000806291800016en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorGök Yurttaş, Asiye
dc.language.isoenen_US
dc.publisherAssociação Médica Brasileiraen_US
dc.relation.ispartofRevista da Associação Médica Brasileiraen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBreast canceren_US
dc.subjectDoxorubicinen_US
dc.subjectMCF-7en_US
dc.subjectOzoneen_US
dc.subjectFibroblasten_US
dc.titleOzone combined with doxorubicin exerts cytotoxic and anticancer effects on Luminal-A subtype human breast cancer cell lineen_US
dc.typeArticleen_US
dspace.entity.typePublication

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