Effects of betulinic acid on AKT/mTOR pathway in renal cell carcinoma
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
AVES
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: Renal cancer is the most lethal among urological cancer. Treatments of renal cell carcinoma (RCC) may be possible by immune checkpoint inhibitors and drug treatment targeting different molecules. We aimed to determine the apoptotic effect of betulinic acid and its effects on expressions of apoptosisassociated genes AKT-1 and mTOR in RCC cells. Material and methods: In this study, we investigated the apoptotic activity of betulinic acid in CAKI-2 cell line and its effect on AKT-1 and mTOR gene expression levels. In order to do so, following analyses were conducted: WST-1 to identify the toxic effect of betulinic acid, Caspase-3/BCA to detect caspase enzyme activity, Annexin-V and ELISA to determine for apoptotic effect, and finally, real-time PCR for expression levels of AKT-1 and mTOR. Results: Our study showed that different concentrations of betulinic acid induced apoptosis in renal cancer; however, no effect was observed in healthy cells. In gene expression analysis, there was statistically significant decrease in AKT-1 expression level while increasing mTOR expression level. Conclusion: We suggested that betulinic acid with its apoptotic effect on RCC line and nontoxic effect on healthy cell line and the effects on AKT/mTOR pathway may be a potential anticancer drug promising for future studies.
Açıklama
Anahtar Kelimeler
Apoptosis, Betulinic Acid, Expression, Pathway, Renal Cancer
Kaynak
Turkish Journal of Urology
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
48
Sayı
1
Künye
Ataş, M.N., Ertuğrul, B., İplik, E.S., Çakmakoğlu, B., Ergen, A. (2022). Effects of betulinic acid on AKT/mTOR pathway in renal cell
carcinoma. Turkish Journal of Urology. 48(1), pp. 66-71. https://10.5152/tud.2022.21276
