Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture
Yükleniyor...
Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Istanbul University
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: A considerable level of evidence has accumulated about the breast cancer risk-reducing effect of consuming specific flavonoids, through the increasing amount of research and epidemiologic studies. Different flavonoids may have different cellular bioavailability and favor, i.e., the occurrence of a hormetic effect, thus it is important to evaluate breast cancer cells’ response to different doses of flavonoids. This study aims to investigate the alterations of the biological pathways in a hormone-positive (HR+) breast cancer cell line as a resemblance for the most common breast cancer subtype, related to the low-dose exposure of the flavonoids. Materials and Methods: Different levels of doses were applied to MCF-7 breast cancer cells. In order to determine cellular proliferation, WST-1 analysis was conducted. The highest proliferation was observed with cell lines exposed to a low-dose flavonoid mixture and these were selected for further analysis. Intracellular protein expression were investigated by peptide analysis on a nano LC-MS/MS platform. A protein-protein interaction network and pathway analysis were conducted for the proteins expressed differently between the groups. Results: A total of 214 proteins were identified and 36 proteins with significant alterations (≥1.2-fold change, p≤0.05) were detected. Significant changes were observed in the pathways related to carbon metabolism, amino acid biosynthesis, splicing mechanism, mitochondrial protein import and translation elongation pathways. Conclusion: Our study demonstrated that flavonoids can have a hormetic effect which can initially alter metabolic pathways vital for cell proliferation and survival. These pathways may include potential targets for enhancing the anticancer activity of the flavonoids
Açıklama
Anahtar Kelimeler
Flavonoids, Breast cancer, Hormesis, Proteomics
Kaynak
European Journal of Biology
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
80
Sayı
2
Künye
Tüzüner, M. B. & Ceviz, A. B. (2021). Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture. European Journal of Biology, 80(2), 145-153. https://doi.org/10.26650/EurJBiol.2021.1017807
