Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture
dc.authorid | 0000-0002-3635-8421 | en_US |
dc.authorscopusid | 57194783843 | en_US |
dc.contributor.author | Tuzuner, Mete Bora | |
dc.contributor.author | Ceviz, Ayşe Begüm | |
dc.date.accessioned | 2022-04-19T06:30:11Z | |
dc.date.available | 2022-04-19T06:30:11Z | |
dc.date.issued | 2021 | en_US |
dc.department | Fakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalı | en_US |
dc.description.abstract | Objective: A considerable level of evidence has accumulated about the breast cancer risk-reducing effect of consuming specific flavonoids, through the increasing amount of research and epidemiologic studies. Different flavonoids may have different cellular bioavailability and favor, i.e., the occurrence of a hormetic effect, thus it is important to evaluate breast cancer cells’ response to different doses of flavonoids. This study aims to investigate the alterations of the biological pathways in a hormone-positive (HR+) breast cancer cell line as a resemblance for the most common breast cancer subtype, related to the low-dose exposure of the flavonoids. Materials and Methods: Different levels of doses were applied to MCF-7 breast cancer cells. In order to determine cellular proliferation, WST-1 analysis was conducted. The highest proliferation was observed with cell lines exposed to a low-dose flavonoid mixture and these were selected for further analysis. Intracellular protein expression were investigated by peptide analysis on a nano LC-MS/MS platform. A protein-protein interaction network and pathway analysis were conducted for the proteins expressed differently between the groups. Results: A total of 214 proteins were identified and 36 proteins with significant alterations (≥1.2-fold change, p≤0.05) were detected. Significant changes were observed in the pathways related to carbon metabolism, amino acid biosynthesis, splicing mechanism, mitochondrial protein import and translation elongation pathways. Conclusion: Our study demonstrated that flavonoids can have a hormetic effect which can initially alter metabolic pathways vital for cell proliferation and survival. These pathways may include potential targets for enhancing the anticancer activity of the flavonoids | en_US |
dc.identifier.citation | Tüzüner, M. B. & Ceviz, A. B. (2021). Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture. European Journal of Biology, 80(2), 145-153. https://doi.org/10.26650/EurJBiol.2021.1017807 | en_US |
dc.identifier.doi | 10.26650/EurJBiol.2021.1017807 | en_US |
dc.identifier.endpage | 153 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.scopus | 2-s2.0-85127704707 | en_US |
dc.identifier.scopusquality | Q4 | en_US |
dc.identifier.startpage | 145 | en_US |
dc.identifier.trdizinid | 507424 | |
dc.identifier.uri | https://doi.org/10.26650/EurJBiol.2021.1017807 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13055/212 | |
dc.identifier.volume | 80 | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | TR-Dizin | en_US |
dc.institutionauthor | Ceviz, Ayşe Begüm | |
dc.language.iso | en | en_US |
dc.publisher | Istanbul University | en_US |
dc.relation.ispartof | European Journal of Biology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Flavonoids | en_US |
dc.subject | Breast cancer | en_US |
dc.subject | Hormesis | en_US |
dc.subject | Proteomics | en_US |
dc.title | Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication |