Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture

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dc.authorid0000-0002-3635-8421en_US
dc.authorscopusid57194783843en_US
dc.contributor.authorTuzuner, Mete Bora
dc.contributor.authorCeviz, Ayşe Begüm
dc.date.accessioned2022-04-19T06:30:11Z
dc.date.available2022-04-19T06:30:11Z
dc.date.issued2021en_US
dc.departmentFakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Tıbbi Genetik Ana Bilim Dalıen_US
dc.description.abstractObjective: A considerable level of evidence has accumulated about the breast cancer risk-reducing effect of consuming specific flavonoids, through the increasing amount of research and epidemiologic studies. Different flavonoids may have different cellular bioavailability and favor, i.e., the occurrence of a hormetic effect, thus it is important to evaluate breast cancer cells’ response to different doses of flavonoids. This study aims to investigate the alterations of the biological pathways in a hormone-positive (HR+) breast cancer cell line as a resemblance for the most common breast cancer subtype, related to the low-dose exposure of the flavonoids. Materials and Methods: Different levels of doses were applied to MCF-7 breast cancer cells. In order to determine cellular proliferation, WST-1 analysis was conducted. The highest proliferation was observed with cell lines exposed to a low-dose flavonoid mixture and these were selected for further analysis. Intracellular protein expression were investigated by peptide analysis on a nano LC-MS/MS platform. A protein-protein interaction network and pathway analysis were conducted for the proteins expressed differently between the groups. Results: A total of 214 proteins were identified and 36 proteins with significant alterations (≥1.2-fold change, p≤0.05) were detected. Significant changes were observed in the pathways related to carbon metabolism, amino acid biosynthesis, splicing mechanism, mitochondrial protein import and translation elongation pathways. Conclusion: Our study demonstrated that flavonoids can have a hormetic effect which can initially alter metabolic pathways vital for cell proliferation and survival. These pathways may include potential targets for enhancing the anticancer activity of the flavonoidsen_US
dc.identifier.citationTüzüner, M. B. & Ceviz, A. B. (2021). Proteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixture. European Journal of Biology, 80(2), 145-153. https://doi.org/10.26650/EurJBiol.2021.1017807en_US
dc.identifier.doi10.26650/EurJBiol.2021.1017807en_US
dc.identifier.endpage153en_US
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85127704707en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage145en_US
dc.identifier.urihttps://doi.org/10.26650/EurJBiol.2021.1017807
dc.identifier.urihttps://hdl.handle.net/20.500.13055/212
dc.identifier.volume80en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorCeviz, Ayşe Begüm
dc.language.isoenen_US
dc.publisherIstanbul Universityen_US
dc.relation.ispartofEuropean Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFlavonoidsen_US
dc.subjectBreast canceren_US
dc.subjectHormesisen_US
dc.subjectProteomicsen_US
dc.titleProteomic analysis revealed underlying biological pathways associated with hormetic response of hormone- positive breast cancer cell line exposed to low-dose flavonoid mixtureen_US
dc.typeArticleen_US
dspace.entity.typePublication

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