Immunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
University of Murcia
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
mTOR is a member of the PI3K/Akt/mTOR signaling pathway that participates in cell growth, proliferation, protein synthesis, transcription, angiogenesis, apoptosis and autophagy. mTOR and MAPK pahways are two important key signal pathways which are related to each other. We investigated the role of mTOR and other signaling molecules in rat ovaries and uteruses in stages of the estrous cycle. Young adult female rats were divided into four groups as proestrous, estrous, metestrous and diestrous according to vaginal smears. Immunohistochemical staining of mTORC1, IGF1, PI3K, pAKT1/2/3, ERK1 and pERK1/2 was performed and pAKT1/2/3 and ERK1 were also analyzed using western blotting on ovarian and uterine tissue samples. According to our results, PI3K/Akt/ mTOR and ERK/pERK showed an increase in the rat ovulation period. When all the groups were evaluated the immunoreactivities for all of the antibodies were detected in the oocytes, granulosa and theca cells, corpus luteum and stroma of ovary and lamina propria, surface and glandular epithelium of uterus with the strongest observed with anti-ERK1 antibody and then with a decreasing trend with anti-mTORC1, anti-pAkt1/2/3, anti-IGF1, anti-PI3K and anti-pERK1/2 antibodies in the proestrus and estrus stages. Differently from other parts of the ovary, highest antibody expression in the corpus luteum was observed in the metestrous stage. Moreover, the existence of pAKT1/2/3 and ERK1 proteins was confirmed with the Western blotting technique. We suggest that mTOR and mTOR-related ERK signaling molecules may participate in the rat ovulation process.
Açıklama
Anahtar Kelimeler
ERK, Estrous Cycle, IGF1, mTORC1, Ovulation, pAKT
Kaynak
Histology and Histopathology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
35
Sayı
11
Künye
Ekizceli, G., İnan, S., Öktem, G., Onur, E., Özbilgin, K. (Nov 2020). Immunohistochemical determination of mTOR pathway molecules in ovaries and uterus in rat estrous cycle stages. Histology and Histopathology, 35(11), pp. 1337-1351. https://doi.org/10.14670/HH-18-258.
