The effects of early postnatal alcohol exposure on bone molecular composition in a mouse model

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dc.authorid0000-0001-7094-3336
dc.authorid0000-0002-9462-0862
dc.authorid0000-0002-7844-6876
dc.contributor.authorDursun, İlknur
dc.contributor.authorElibol, Birsen
dc.contributor.authorGarip Ustaoğlu, Şebnem
dc.date.accessioned2026-06-08T13:02:31Z
dc.date.available2026-06-08T13:02:31Z
dc.date.issued2026
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Fizyoloji Ana Bilim Dalı
dc.description.abstractBackground: Perinatal exposure to alcohol is a critical risk factor for long- term skeletal health in humans and has been demon strated in rodent models. Early postnatal alcohol gavage exposure in mice is used to study third trimester toxicity in humans. However, further research is required to fully characterize the effects of alcohol on bone molecular composition and distinguish them from the alterations associated with gavage- related stress in mouse models. Methods: Female mouse pups were assigned to untreated control (C), gavage control (GC), and alcohol- treated (A) groups. Between postnatal days 3 and 20, the A group received ethanol (3.0 g/kg/day) by intragastric gavage, while the GC group under went the same gavage procedure without any solution (neither milk nor ethanol). At postnatal day 90, femoral bone molecular composition and bone metabolism were evaluated by attenuated total reflection Fourier- transform infrared (ATR- FTIR) spec troscopy and biochemical studies. Results: Several ATR- FTIR- derived matrix and mineral- related alterations, including reduced total protein- related indices, lower collagen cross- link ratio, reduced mineral- to- matrix ratio, and increased crystallinity, were observed in both GC and A groups rel ative to untreated controls, indicating a substantial long- term influence of the gavage procedure itself. Compared with the GC, the A group showed additional selective differences, most notably reduced relative carbonate content and increased CTX- I levels. In the C group, serum 25(OH)D, PTH, and calcitonin levels differed from both GC and A groups, whereas osteocalcin was increased in the GC group relative to the C group, and no significant difference was observed between the GC and A groups. Conclusion: Prolonged neonatal gavage was associated with substantial long- term changes in bone molecular composition and several circulating bone- related hormonal markers. Against this background, postnatal alcohol exposure was associated with additional selective differences, particularly in CTX- I and relative carbonate content. These findings highlight the importance of discriminating between the effect of the procedure and the effect of alcohol in developmental models based on repeated neonatal gavage.
dc.description.sponsorshipThis work is part of a research project funded by Altinbas University, Research Council, (number: PB2022- TIP- 3). Bu çalışma, Altınbaş Üniversitesi Araştırma Konseyi tarafından finanse edilen bir araştırma projesinin (numara: PB2022- TIP- 3) bir parçasıdır.
dc.identifier.citationDursun, İ., Elibol, B., & Garip Ustaoğlu, Ş. (2026). The effects of early postnatal alcohol exposure on bone molecular composition in a mouse model. Alcohol, Clinical and Experimental Research, 50(6), pp. 1-11. https://doi.org/10.1111/acer.70331
dc.identifier.doi10.1111/acer.70331
dc.identifier.endpage11
dc.identifier.issn2993-7175
dc.identifier.issue6
dc.identifier.pmidPMID: 42246483
dc.identifier.scopusqualityQ2
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1111/acer.70331
dc.identifier.urihttps://hdl.handle.net/20.500.13055/1510
dc.identifier.volume50
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expanded
dc.institutionauthorDursun, İlknur
dc.institutionauthorid0000-0001-7094-3336
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofAlcohol, Clinical and Experimental Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectATR- FTIR Spectroscopy
dc.subjectBone
dc.subjectBone Metabolism
dc.subjectBone Quality Spectral Markers
dc.subjectBone- Related Hormonal Markers
dc.subjectGavage- Related Stress
dc.subjectPostnatal Alcohol Exposure
dc.titleThe effects of early postnatal alcohol exposure on bone molecular composition in a mouse model
dc.typeArticle
dspace.entity.typePublication

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