The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats
dc.authorid | 0000-0002-6424-7411 | en_US |
dc.authorscopusid | 57366670900 | en_US |
dc.contributor.author | Daşçı, Mustafa Fatih | |
dc.contributor.author | Yaprak Saraç, Elif | |
dc.contributor.author | Gök Yurttaş, Asiye | |
dc.contributor.author | Atçı, Tolgahan | |
dc.contributor.author | Uslu, Muhammed | |
dc.contributor.author | Acar, Abdurrahman | |
dc.contributor.author | Güleç, Mehmet Akif | |
dc.contributor.author | Alagöz, Ender | |
dc.date.accessioned | 2022-11-29T06:41:37Z | |
dc.date.available | 2022-11-29T06:41:37Z | |
dc.date.issued | 2022 | en_US |
dc.department | Fakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Biyokimya Ana Bilim Dalı | en_US |
dc.description.abstract | Objectives: In this study, we aimed to evaluate the antioxidant, antiapoptotic, osteoblastic and hypolipidemic effects of thymoquinone (TQ) treatment on the steroid-induced osteonecrosis of femoral head (ONFH) model in rats. Materials and methods: A total of 24 rats were randomly divided into four groups: the control group administered saline; the TQ group administered 10 mg/kg/day TQ orally; lipopolysaccharide/ methylprednisolone (LPS/MPS) group administered 20 μg/kg intraperitoneally LPS and 40 mg/kg intramuscularly MPS to establish ONFH model; and the LPS/MPS+TQ group administered both LPS/MPS and, then, TQ once daily for four weeks. All rats were sacrificed after intracardiac blood collection and their right femurs were removed. Results: Micro-computed tomography showed a higher bone mineral density and lower porosity, Tr.Sp and Tr.Sep data were detected in the LPS/MPS+TQ group. In histopathology, osteonecrosis increased significantly in the LPS/MPS group and osteonecrosis decreased in the LPS/MPS+TQ group compared to the LPS/MPS group (p=0.0077). Histomorphometric examination revealed that the percentage of BV/TV in the LPS/MPS group was significantly lower compared to control and other groups (p<0.01 and p<0.05, respectively), while it reached normal rates in the LPS/MPS+TQ group. Immunohistochemically, antioxidant, anti-apoptotic, and angiogenesis indicators (8-hydroxy-20-deoxyguanosine [8-OHdG], malondialdehyde [MDA], B-cell lymphoma [Bcl-2], caspase-3, vascular endothelial growth factor [VEGF]) were significantly improved in tissue and serum with TQ. Furthermore, TQ significantly reduced low and high-density lipoprotein cholesterol ratio and carboxy-terminal type 1 collagen crosslink (CTX) in serum. Conclusion: Vascular and hematopoietic cell damages that occur due to steroid-induced deoxyribonucleic acid (DNA) oxidative and lipid peroxidative damages in an ONFH model can be successfully ameliorated by TQ administration. This antioxidant and antiapoptotic effects of TQ may be a promising treatment option for early stage of osteonecrosis. | en_US |
dc.identifier.citation | Daşçı, M. F., Yaprak Saraç, E., Gök Yurttaş, A., Atçı, T., Uslu, M., Acar, A. ... Alagöz, E. (2022). The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats. Joint Diseases and Related Surgery, 33(3), pp. 553-566. https://doi.org/10.52312/jdrs.2022.752 | en_US |
dc.identifier.doi | 10.52312/jdrs.2022.752 | en_US |
dc.identifier.endpage | 566 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.pmid | PMID: 36345183 | en_US |
dc.identifier.scopus | 2-s2.0-85141772028 | en_US |
dc.identifier.scopusquality | Q2 | en_US |
dc.identifier.startpage | 553 | en_US |
dc.identifier.trdizinid | 1176316 | |
dc.identifier.uri | https://doi.org/10.52312/jdrs.2022.752 | |
dc.identifier.uri | https://hdl.handle.net/20.500.13055/322 | |
dc.identifier.volume | 33 | en_US |
dc.identifier.wos | WOS:000876959700001 | en_US |
dc.identifier.wosquality | Q4 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | TR-Dizin | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.indekslendigikaynak.other | SCI-E - Science Citation Index Expanded | en_US |
dc.institutionauthor | Gök Yurttaş, Asiye | |
dc.language.iso | en | en_US |
dc.publisher | Turkish Joint Diseases Foundation | en_US |
dc.relation.ispartof | Joint Diseases and Related Surgery | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Lipopolysaccharide | en_US |
dc.subject | Methylprednisolone | en_US |
dc.subject | Osteonecrosis of Femoral Head | en_US |
dc.subject | Thymoquinone | en_US |
dc.title | The effects of thymoquinone on steroid-induced femoral head osteonecrosis: An experimental study in rats | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication |