Determination of Rh type and gender using circulating cell-free fetal DNA in early pregnancy of Rh negative women in Turkey
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Introduction: Choosing the right clinical approach for early and reliable diagnosis/screening is becoming more important day by day. The aim of the study was to determine the early RhD type with cff-DNA obtained from maternal plasma, especially in the light of recent developments. In this way, it is aimed to apply Rh Ig only to mothers who are determined to have RhD (+) fetuses and to prevent unnecessary further tests that may possess a risk for RhD (-) fetuses. Methods: Prediction of fetal gender and RH genotype was performed by using RT-qPCR method. With simultaneous amplification of sequences of SRY, DYS14 and RH genes (exon 7 and exon 10). Fetal gender and RhD were determined in 30 RHD (-) pregnant women with cfDNA. Results: As a result of genotyping, the gender of 67% (20/30) fetuses was determined as male; the gender of 33% (10/30) fetuses was determined as female in a sample group of 30 pregnancies. It was determined that the DYS14 100% (20/20) gene was more sensitive than the SRY 97% (18/20) gene in gender determination after examining prenatal and postnatal results. As a result of the analysis, the presence of 17% (5/30) RhD (-) fetuses and 83% (25/30) RhD (+) fetuses were determined which is 100% compatible with postnatal results. Discussion: Detecting fetal RhD gene in maternal plasma made an important contribution to its use in non-invasive prenatal screening. This study shows that unnecessary intervention and cost can be avoided with successful genotyping analysis performed with RT-qPCR.
Açıklama
Anahtar Kelimeler
DYS14, Non-Invasive Prenatal Diagnosis, RHD Type, Real-Time PCR, Cff-DNA
Kaynak
Transfusion Clinique et Biologique
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
30
Sayı
3
Künye
Elgün, T., Müşteri Oltulu, Y., Yurttaş Gök, A., Aygüz, U., & Kılıç, U. (2023). Determination of Rh type and gender using circulating cell-free fetal DNA in early pregnancy of Rh negative women in Turkey. Transfusion Clinique et Biologique, 30(3), pp. 324-328. https://doi.org/10.1016/j.tracli.2023.04.004
