Determination of Rh type and gender using circulating cell-free fetal DNA in early pregnancy of Rh negative women in Turkey

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dc.authorid0000-0002-6424-7411en_US
dc.authorscopusid57366670900en_US
dc.authorwosidCJT-9277-2022en_US
dc.contributor.authorElgün, Tuğba
dc.contributor.authorMüşteri Oltulu, Yasemin
dc.contributor.authorYurttaş Gök, Asiye
dc.contributor.authorAygüz, Umut
dc.contributor.authorKılıç, Ulkan
dc.date.accessioned2023-04-30T13:34:27Z
dc.date.available2023-04-30T13:34:27Z
dc.date.issued2023en_US
dc.departmentFakülteler, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümü, Biyokimya Ana Bilim Dalıen_US
dc.description.abstractIntroduction: Choosing the right clinical approach for early and reliable diagnosis/screening is becoming more important day by day. The aim of the study was to determine the early RhD type with cff-DNA obtained from maternal plasma, especially in the light of recent developments. In this way, it is aimed to apply Rh Ig only to mothers who are determined to have RhD (+) fetuses and to prevent unnecessary further tests that may possess a risk for RhD (-) fetuses. Methods: Prediction of fetal gender and RH genotype was performed by using RT-qPCR method. With simultaneous amplification of sequences of SRY, DYS14 and RH genes (exon 7 and exon 10). Fetal gender and RhD were determined in 30 RHD (-) pregnant women with cfDNA. Results: As a result of genotyping, the gender of 67% (20/30) fetuses was determined as male; the gender of 33% (10/30) fetuses was determined as female in a sample group of 30 pregnancies. It was determined that the DYS14 100% (20/20) gene was more sensitive than the SRY 97% (18/20) gene in gender determination after examining prenatal and postnatal results. As a result of the analysis, the presence of 17% (5/30) RhD (-) fetuses and 83% (25/30) RhD (+) fetuses were determined which is 100% compatible with postnatal results. Discussion: Detecting fetal RhD gene in maternal plasma made an important contribution to its use in non-invasive prenatal screening. This study shows that unnecessary intervention and cost can be avoided with successful genotyping analysis performed with RT-qPCR.en_US
dc.identifier.citationElgün, T., Müşteri Oltulu, Y., Yurttaş Gök, A., Aygüz, U., & Kılıç, U. (2023). Determination of Rh type and gender using circulating cell-free fetal DNA in early pregnancy of Rh negative women in Turkey. Transfusion Clinique et Biologique, 30(3), pp. 324-328. https://doi.org/10.1016/j.tracli.2023.04.004en_US
dc.identifier.doi10.1016/j.tracli.2023.04.004en_US
dc.identifier.endpage328en_US
dc.identifier.issn1246-7820
dc.identifier.issue3en_US
dc.identifier.pmidPMID: 37116742en_US
dc.identifier.scopus2-s2.0-85159097981en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage324en_US
dc.identifier.urihttps://doi.org/10.1016/j.tracli.2023.04.004
dc.identifier.urihttps://hdl.handle.net/20.500.13055/454
dc.identifier.volume30en_US
dc.identifier.wosWOS:001047988600001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.indekslendigikaynak.otherSCI-E - Science Citation Index Expandeden_US
dc.institutionauthorYurttaş Gök, Asiye
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTransfusion Clinique et Biologiqueen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDYS14en_US
dc.subjectNon-Invasive Prenatal Diagnosisen_US
dc.subjectRHD Typeen_US
dc.subjectReal-Time PCRen_US
dc.subjectCff-DNAen_US
dc.titleDetermination of Rh type and gender using circulating cell-free fetal DNA in early pregnancy of Rh negative women in Turkeyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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